PhytophthoraV1, Main, Exploration, bibRecord, 000E62

Functional Divergence of Two Secreted Immune Proteases of Tomato.

Identifieur interne : 000E62 ( Main/Exploration ); précédent : 000E61; suivant : 000E63

Functional Divergence of Two Secreted Immune Proteases of Tomato.

Auteurs : Muhammad Ilyas [Allemagne] ; Anja C. Hörger [Allemagne] ; Tolga O. Bozkurt [Royaume-Uni] ; Harrold A. Van Den Burg [Pays-Bas] ; Farnusch Kaschani [Allemagne] ; Markus Kaiser [Allemagne] ; Khaoula Belhaj [Royaume-Uni] ; Matthew Smoker [Royaume-Uni] ; Matthieu H A J. Joosten [Pays-Bas] ; Sophien Kamoun [Royaume-Uni] ; Renier A L. Van Der Hoorn [Allemagne]

Source :

Current biology : CB [ 1879-0445 ] ; 2015.

RBID : pubmed:26299516

Descripteurs français

KwdFr :
- Cladosporium (physiologie), Duplication de gène (MeSH), Lycopersicon esculentum (génétique), Lycopersicon esculentum (immunologie), Lycopersicon esculentum (métabolisme), Peptide hydrolases (génétique), Peptide hydrolases (métabolisme), Phytophthora infestans (physiologie), Protéines végétales (génétique), Protéines végétales (métabolisme), Évolution moléculaire (MeSH).
MESH :
- génétique : Lycopersicon esculentum, Peptide hydrolases, Protéines végétales.
- immunologie : Lycopersicon esculentum.
- métabolisme : Lycopersicon esculentum, Peptide hydrolases, Protéines végétales.
- physiologie : Cladosporium, Phytophthora infestans.
- Duplication de gène, Évolution moléculaire.

English descriptors

KwdEn :
- Cladosporium (physiology), Evolution, Molecular (MeSH), Gene Duplication (MeSH), Lycopersicon esculentum (genetics), Lycopersicon esculentum (immunology), Lycopersicon esculentum (metabolism), Peptide Hydrolases (genetics), Peptide Hydrolases (metabolism), Phytophthora infestans (physiology), Plant Proteins (genetics), Plant Proteins (metabolism).
MESH :
- chemical , genetics : Peptide Hydrolases, Plant Proteins.
- genetics : Lycopersicon esculentum.
- immunology : Lycopersicon esculentum.
- metabolism : Lycopersicon esculentum, Peptide Hydrolases, Plant Proteins.
- physiology : Cladosporium, Phytophthora infestans.
- Evolution, Molecular, Gene Duplication.

Abstract

Rcr3 and Pip1 are paralogous secreted papain-like proteases of tomato. Both proteases are inhibited by Avr2 from the fungal pathogen Cladosporium fulvum, but only Rcr3 acts as a co-receptor for Avr2 recognition by the tomato Cf-2 immune receptor. Here, we show that Pip1-depleted tomato plants are hyper-susceptible to fungal, bacterial, and oomycete plant pathogens, demonstrating that Pip1 is an important broad-range immune protease. By contrast, in the absence of Cf-2, Rcr3 depletion does not affect fungal and bacterial infection levels but causes increased susceptibility only to the oomycete pathogen Phytophthora infestans. Rcr3 and Pip1 reside on a genetic locus that evolved over 36 million years ago. These proteins differ in surface-exposed residues outside the substrate-binding groove, and Pip1 is 5- to 10-fold more abundant than Rcr3. We propose a model in which Rcr3 and Pip1 diverged functionally upon gene duplication, possibly driven by an arms race with pathogen-derived inhibitors or by coevolution with the Cf-2 immune receptor detecting inhibitors of Rcr3, but not of Pip1.

DOI: 10.1016/j.cub.2015.07.030
PubMed: 26299516

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000D81
to stream Main, to step Curation: 000D81

Le document en format XML

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<country xml:lang="fr">Allemagne</country>
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<author><name sortKey="Horger, Anja C" sort="Horger, Anja C" uniqKey="Horger A" first="Anja C" last="Hörger">Anja C. Hörger</name>
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<author><name sortKey="Kaiser, Markus" sort="Kaiser, Markus" uniqKey="Kaiser M" first="Markus" last="Kaiser">Markus Kaiser</name>
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<country xml:lang="fr">Allemagne</country>
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<author><name sortKey="Belhaj, Khaoula" sort="Belhaj, Khaoula" uniqKey="Belhaj K" first="Khaoula" last="Belhaj">Khaoula Belhaj</name>
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<country xml:lang="fr">Royaume-Uni</country>
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<author><name sortKey="Smoker, Matthew" sort="Smoker, Matthew" uniqKey="Smoker M" first="Matthew" last="Smoker">Matthew Smoker</name>
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<country xml:lang="fr">Royaume-Uni</country>
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<author><name sortKey="Joosten, Matthieu H A J" sort="Joosten, Matthieu H A J" uniqKey="Joosten M" first="Matthieu H A J" last="Joosten">Matthieu H A J. Joosten</name>
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<country xml:lang="fr" wicri:curation="lc">Pays-Bas</country>
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<author><name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name>
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<country xml:lang="fr">Royaume-Uni</country>
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<country xml:lang="fr">Allemagne</country>
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<term>Evolution, Molecular (MeSH)</term>
<term>Gene Duplication (MeSH)</term>
<term>Lycopersicon esculentum (genetics)</term>
<term>Lycopersicon esculentum (immunology)</term>
<term>Lycopersicon esculentum (metabolism)</term>
<term>Peptide Hydrolases (genetics)</term>
<term>Peptide Hydrolases (metabolism)</term>
<term>Phytophthora infestans (physiology)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Cladosporium (physiologie)</term>
<term>Duplication de gène (MeSH)</term>
<term>Lycopersicon esculentum (génétique)</term>
<term>Lycopersicon esculentum (immunologie)</term>
<term>Lycopersicon esculentum (métabolisme)</term>
<term>Peptide hydrolases (génétique)</term>
<term>Peptide hydrolases (métabolisme)</term>
<term>Phytophthora infestans (physiologie)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Évolution moléculaire (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptide Hydrolases</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lycopersicon esculentum</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Lycopersicon esculentum</term>
<term>Peptide hydrolases</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lycopersicon esculentum</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Lycopersicon esculentum</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lycopersicon esculentum</term>
<term>Peptide Hydrolases</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lycopersicon esculentum</term>
<term>Peptide hydrolases</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cladosporium</term>
<term>Phytophthora infestans</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cladosporium</term>
<term>Phytophthora infestans</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Evolution, Molecular</term>
<term>Gene Duplication</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Duplication de gène</term>
<term>Évolution moléculaire</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Rcr3 and Pip1 are paralogous secreted papain-like proteases of tomato. Both proteases are inhibited by Avr2 from the fungal pathogen Cladosporium fulvum, but only Rcr3 acts as a co-receptor for Avr2 recognition by the tomato Cf-2 immune receptor. Here, we show that Pip1-depleted tomato plants are hyper-susceptible to fungal, bacterial, and oomycete plant pathogens, demonstrating that Pip1 is an important broad-range immune protease. By contrast, in the absence of Cf-2, Rcr3 depletion does not affect fungal and bacterial infection levels but causes increased susceptibility only to the oomycete pathogen Phytophthora infestans. Rcr3 and Pip1 reside on a genetic locus that evolved over 36 million years ago. These proteins differ in surface-exposed residues outside the substrate-binding groove, and Pip1 is 5- to 10-fold more abundant than Rcr3. We propose a model in which Rcr3 and Pip1 diverged functionally upon gene duplication, possibly driven by an arms race with pathogen-derived inhibitors or by coevolution with the Cf-2 immune receptor detecting inhibitors of Rcr3, but not of Pip1.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26299516</PMID>
<DateCompleted><Year>2016</Year>
<Month>06</Month>
<Day>07</Day>
</DateCompleted>
<DateRevised><Year>2015</Year>
<Month>09</Month>
<Day>02</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0445</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>25</Volume>
<Issue>17</Issue>
<PubDate><Year>2015</Year>
<Month>Aug</Month>
<Day>31</Day>
</PubDate>
</JournalIssue>
<Title>Current biology : CB</Title>
<ISOAbbreviation>Curr Biol</ISOAbbreviation>
</Journal>
<ArticleTitle>Functional Divergence of Two Secreted Immune Proteases of Tomato.</ArticleTitle>
<Pagination><MedlinePgn>2300-6</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cub.2015.07.030</ELocationID>
<ELocationID EIdType="pii" ValidYN="Y">S0960-9822(15)00833-7</ELocationID>
<Abstract><AbstractText>Rcr3 and Pip1 are paralogous secreted papain-like proteases of tomato. Both proteases are inhibited by Avr2 from the fungal pathogen Cladosporium fulvum, but only Rcr3 acts as a co-receptor for Avr2 recognition by the tomato Cf-2 immune receptor. Here, we show that Pip1-depleted tomato plants are hyper-susceptible to fungal, bacterial, and oomycete plant pathogens, demonstrating that Pip1 is an important broad-range immune protease. By contrast, in the absence of Cf-2, Rcr3 depletion does not affect fungal and bacterial infection levels but causes increased susceptibility only to the oomycete pathogen Phytophthora infestans. Rcr3 and Pip1 reside on a genetic locus that evolved over 36 million years ago. These proteins differ in surface-exposed residues outside the substrate-binding groove, and Pip1 is 5- to 10-fold more abundant than Rcr3. We propose a model in which Rcr3 and Pip1 diverged functionally upon gene duplication, possibly driven by an arms race with pathogen-derived inhibitors or by coevolution with the Cf-2 immune receptor detecting inhibitors of Rcr3, but not of Pip1.</AbstractText>
<CopyrightInformation>Copyright © 2015 Elsevier Ltd. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ilyas</LastName>
<ForeName>Muhammad</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Plant Chemetics Laboratory, Max Planck Institute for Plant Breeding Research, Carl-von-Linné Weg 10, 50829 Cologne, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hörger</LastName>
<ForeName>Anja C</ForeName>
<Initials>AC</Initials>
<AffiliationInfo><Affiliation>Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB Oxford, UK; Plant Chemetics Laboratory, Max Planck Institute for Plant Breeding Research, Carl-von-Linné Weg 10, 50829 Cologne, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bozkurt</LastName>
<ForeName>Tolga O</ForeName>
<Initials>TO</Initials>
<AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>van den Burg</LastName>
<ForeName>Harrold A</ForeName>
<Initials>HA</Initials>
<AffiliationInfo><Affiliation>Molecular Plant Pathology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, the Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kaschani</LastName>
<ForeName>Farnusch</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Chemical Biology, Fakultät für Biologie, Zentrum für Medizinische Biotechnologie, Universität Duisburg-Essen, Universitätstr. 2, 45117 Essen, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kaiser</LastName>
<ForeName>Markus</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Chemical Biology, Fakultät für Biologie, Zentrum für Medizinische Biotechnologie, Universität Duisburg-Essen, Universitätstr. 2, 45117 Essen, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Belhaj</LastName>
<ForeName>Khaoula</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Smoker</LastName>
<ForeName>Matthew</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Joosten</LastName>
<ForeName>Matthieu H A J</ForeName>
<Initials>MH</Initials>
<AffiliationInfo><Affiliation>SOL Group, Laboratory of Phytopathology, Droevendaalsesteeg 1, 6708 PB Wageningen, the Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kamoun</LastName>
<ForeName>Sophien</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>van der Hoorn</LastName>
<ForeName>Renier A L</ForeName>
<Initials>RA</Initials>
<AffiliationInfo><Affiliation>Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB Oxford, UK; Plant Chemetics Laboratory, Max Planck Institute for Plant Breeding Research, Carl-von-Linné Weg 10, 50829 Cologne, Germany. Electronic address: renier.vanderhoorn@plants.ox.ac.uk.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2015</Year>
<Month>08</Month>
<Day>20</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Curr Biol</MedlineTA>
<NlmUniqueID>9107782</NlmUniqueID>
<ISSNLinking>0960-9822</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.-</RegistryNumber>
<NameOfSubstance UI="D010447">Peptide Hydrolases</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D002963" MajorTopicYN="N">Cladosporium</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019143" MajorTopicYN="Y">Evolution, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020440" MajorTopicYN="N">Gene Duplication</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010447" MajorTopicYN="N">Peptide Hydrolases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cladosporium fulvum</Keyword>
<Keyword MajorTopicYN="N">Cys protease</Keyword>
<Keyword MajorTopicYN="N">Phytophthora infestans</Keyword>
<Keyword MajorTopicYN="N">apoplast</Keyword>
<Keyword MajorTopicYN="N">immunity</Keyword>
<Keyword MajorTopicYN="N">tomato</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year>
<Month>05</Month>
<Day>29</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2015</Year>
<Month>06</Month>
<Day>19</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2015</Year>
<Month>07</Month>
<Day>10</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2015</Year>
<Month>8</Month>
<Day>25</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2015</Year>
<Month>8</Month>
<Day>25</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2016</Year>
<Month>6</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">26299516</ArticleId>
<ArticleId IdType="pii">S0960-9822(15)00833-7</ArticleId>
<ArticleId IdType="doi">10.1016/j.cub.2015.07.030</ArticleId>
</ArticleIdList>
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</pubmed>
<affiliations><list><country><li>Allemagne</li>
<li>Pays-Bas</li>
<li>Royaume-Uni</li>
</country>
<region><li>District de Cologne</li>
<li>Hollande-Septentrionale</li>
<li>Rhénanie-du-Nord-Westphalie</li>
</region>
<settlement><li>Amsterdam</li>
<li>Cologne</li>
</settlement>
<orgName><li>Université d'Amsterdam</li>
<li>Université d'Oxford</li>
</orgName>
</list>
<tree><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Ilyas, Muhammad" sort="Ilyas, Muhammad" uniqKey="Ilyas M" first="Muhammad" last="Ilyas">Muhammad Ilyas</name>
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<name sortKey="Horger, Anja C" sort="Horger, Anja C" uniqKey="Horger A" first="Anja C" last="Hörger">Anja C. Hörger</name>
<name sortKey="Kaiser, Markus" sort="Kaiser, Markus" uniqKey="Kaiser M" first="Markus" last="Kaiser">Markus Kaiser</name>
<name sortKey="Kaschani, Farnusch" sort="Kaschani, Farnusch" uniqKey="Kaschani F" first="Farnusch" last="Kaschani">Farnusch Kaschani</name>
<name sortKey="Van Der Hoorn, Renier A L" sort="Van Der Hoorn, Renier A L" uniqKey="Van Der Hoorn R" first="Renier A L" last="Van Der Hoorn">Renier A L. Van Der Hoorn</name>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Bozkurt, Tolga O" sort="Bozkurt, Tolga O" uniqKey="Bozkurt T" first="Tolga O" last="Bozkurt">Tolga O. Bozkurt</name>
</noRegion>
<name sortKey="Belhaj, Khaoula" sort="Belhaj, Khaoula" uniqKey="Belhaj K" first="Khaoula" last="Belhaj">Khaoula Belhaj</name>
<name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name>
<name sortKey="Smoker, Matthew" sort="Smoker, Matthew" uniqKey="Smoker M" first="Matthew" last="Smoker">Matthew Smoker</name>
</country>
<country name="Pays-Bas"><region name="Hollande-Septentrionale"><name sortKey="Van Den Burg, Harrold A" sort="Van Den Burg, Harrold A" uniqKey="Van Den Burg H" first="Harrold A" last="Van Den Burg">Harrold A. Van Den Burg</name>
</region>
<name sortKey="Joosten, Matthieu H A J" sort="Joosten, Matthieu H A J" uniqKey="Joosten M" first="Matthieu H A J" last="Joosten">Matthieu H A J. Joosten</name>
</country>
</tree>
</affiliations>
</record>

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Functional Divergence of Two Secreted Immune Proteases of Tomato.

Functional Divergence of Two Secreted Immune Proteases of Tomato.

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